Here you can take a closer look on what we have been up to and when. Just hover over dates to see date specific tasks and click on + sign for more information!
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12 | 13 | 14 / 12 / 2022Talked about what iGEM is, what last year’s team did, what are the next steps in this project and then the team members got to know each other a little bit. | 15 | 16 | 17 | 18 |
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9 | 10 | 11 / 01 / 2023Held first official team meeting where we selected team leaders, went through some practicalities and started brainstorming. | 12 | 13 | 14 | 15 |
16 | 17 | 18 | 19 | 20 | 21 | 22 |
23 | 24 / 01 / 2023We discussed office and work spaces, met a new team member and started brainstorming. | 25 | 26 | 27 | 28 | 29 |
30 | 31 / 01 / 2023We decided sub team leaders for human practices, dry lab, wet lab and IT, talked about each group's responsibilities and met yet another new team member. |
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6 | 7 | 8 | 09 / 02 / 2023First group photo of the team and a caption that says we are excited for this iGEM journey. | 10 | 11 | 12 |
13 | 14 / 02 / 2023We discussed this year’s team logo, making of one-pager and upcoming presentation for Suomen bioteollisuus ry. Started to vision our team values, talked about Bioteollisuus Forum presentation, looked at the medal criteria and started to plan our education journey | 15 | 16 | 17 | 18 | 19 |
20 | 21 | 22 | 23 | 24 | 25 | 26 / 06 / 2023Post about our logo with the new team colors that Jesse picked out (team voted which combo is the best). Quote from the caption: We wanted to carry on the tradition where the new ABOA team picks out their own new colors for the team logo! |
27 | 28 / 02 / 2023We agreed on future meeting time, what medals we would like to aim for and talked about labs. |
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06 / 03 / 2023Mariel and Ami went to present ABOA for 15 min to second-year biochemistry students to promote ABOA, iGEM and recruit new team members. | 07 / 03 / 2023We agreed on time for a team photoshoot and brainstormed for professionals to contact about our project idea. | 8 | 09/ 03 / 2023Meeting with our PI to talk about the project topics we have been thinking about. We narrowed down the possible topics to a few of them. | 10 / 03 / 2023Planned fundraising strategy, wrote an email base and set responsibilities to each subteam member. | 11 / 03 / 2023Sara and Mariel went to have a cup of coffee and tea. We discussed what should be done and when. Basic team administration. | 12 |
13 / 03 / 2023Sara and Saana went to present ABOA for 15 min to third-year students to promote ABOA, iGEM and recruit team members. We got two team members after this!! | 14 / 03 / 2023We took our first professional group photos and individual photos at Quantum. After the photoshoot we went to a pub to hangout. | 15 / 03 / 2023We met our new group members from Åbo Akademi, talked about starting a promotion video and discussed lab status. | 16 | 17 | 18 | 19 |
20 | 21 / 03 / 2023Talked about fundraising status, future school visits and what researchers have been contacted. | 22 | 23 / 03 / 2023Promo video planning, decided how to execute the individual introductions on Instagram and updated our social media accounts. We introduced every team member on Instagram: their name, what they study, what sub teams they are part of and favorite things. | 24 | 25 | 26 |
27 | 28 / 03 / 2023We talked with Aino, who is a preschool teacher at Ilpoisten päiväkoti, to talk about how we should teach the children about synthetic biology. | 29 / 03 / 2023Satu and Joakim had a meeting with Jasper. They visited the lab, the warehouse and talked about the lab work in general. Went through what lab teams are doing, what is the budget (750€) and agreed on team values. | 30 | 31 / 03 / 2023Participated in a World Health Day collaboration that was organized by the Patras Medicine iGEM team. We made and decorated a poster about Joyce Meyer’s quote. We sent a photo of us with the poster to Patras Medicine and they made an Instagram compilation of iGEM teams who participated. |
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03 / 04 / 2023Sara and Ami held a presentation about ABOA and our project. We got a grant from the University of Turku after this meeting. | 04 / 04 / 2023Talked about fundraising status, future school visits and team shirts. We sent fundraising emails to companies together and applied for grants. | 5 | 6 | 7 | 8 | 9 |
10 | 11 / 04 / 2023We visited Ilpoisten päiväkoti and taught 3- to 5-year-old children about lab safety and cell modification. | 12 / 04 / 2023Talked about collaboration with the Aalto-Helsinki team and arranging a meeting with them. Discussed also about our research plan and team registration. We brainstormed promo video ideas and decided the plan and schedule for the execution. | 13 / 04 / 2023We discussed how to do the team registration. | 14 / 04 / 2023Talked overall about Synechocystis, what things need to be considered when growing cyanobacteria, how to monitor cell functions and how to handle mercury in the lab. | 15 | 16 |
17 | 18 / 04 / 2023In honor of Earth Day we participated in a Clean Up collaboration that was organized by the iGEM Bulgaria team. We cleaned up the area near our campuses. Went through IT, media and lab statuses, talked about registration and made a plan for summer. | 19 / 04 / 2023We had an opportunity to talk about synthetic biology, our project and iGEM in general in the Bioteollisuus Forum that was organized by Finnish Bioindustries. Before our presentation we learn about the current situation of synthetic biology in Finland and the different Finnish companies in this field. After the presentations we had the chance to have eye-opening conversations with the representatives of various bioindustry companies and organizations. | 20 | 21 | 22 | 23 |
24 | 25 / 04 / 2023Sara and Mariel had a meeting with our PI. They registered the team for the iGEM competition and discussed how to proceed. They also got some very good advice as team leaders from the PI. | 26 | 27 | 28 | 29 | 30 |
Mon | Tue | Wed | Thu | Fri | Sat | Sun |
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1 | 02 / 05 / 2023Went through our current budget, upcoming events and lab team got everyone up to date on what was happening. | 3 | 04 / 05 / 2023Discussed the promo video and its schedule, started to plan our education campaign and decided upon our project reveal. Discussed how to get started with lab, research and what things need to be considered in the different phases of the project. | 5 | 6 | 7 |
8 | 09 / 05 / 2023We started our educational campaign on Instagram. We explained different synthetic biology terms and topics regarding our projects. We had a Zoom meeting with the other Finnish team: Aalto-Helsinki. We presented our project ideas and talked about collaboration ideas. | 10 / 05 / 2023Talked about upcoming lab orientation, collaboration status with the Aalto-Helsinki team and project status. Discussed our interest in Turku’s Comic Festival, decided the plan for Tiedekoulu visit, planned our future events and compiled a list of questions that you help start the conversations with our stakeholders. | 11 | 12 / 05 / 2023Visited an upper secondary school in Helsinki called Kulosaaren yhteiskoulu. We taught them about our project, iGEM and different biology terms especially those regarding genetically modified organisms. To activate the audience and get the conversation following, the students got to brainstorm their own iGEM project and we went through those together in the classroom. | 13 | 14 |
15 / 05 / 2023Everyone who wanted to work in the lab during the summer needed to participate in a laboratory orientation which Anniina Lepistö held in order to ensure the safety of us and others. We walked through the different lab facilities, learned protocols regarding autoclave and other devices, learned about the break room etiquette (if you drink coffee, you bring coffee) and said hi to various people working in the lab. We visited Turku’s Tiedekoulu (Science school in English) and there we taught the children about cells, microscopes and together we extracted DNA from bananas. | 16 / 05 / 2023We had a meeting with the subteam leaders to check that everything is already for the upcoming summer months. Talked about the Biotop meeting, project name and future collaborations and upcoming events. | 17 / 05 / 2023Went over research to decide the genes we would be working with. Then started to design the genes and the construct. | 18 | 19 | 20 | 21 |
22 / 05 / 2023Went over what progress has been made and how we should continue. | 23 / 05 / 2023Had a meeting with Aalto-Helsinki and Heureka. We discussed the possible workshops and dates for the education events. | 24 / 05 / 2023We planned our future Human practice work and education journey: we planned how to organize | 25 / 05 / 2023We discussed what are the sources and impacts of mercury in aquatic ecosystems. We discussed forestry, its history and future, how forestry is done on marshlands. We discussed ditching of marshlands and what are its impacts on forests, ecosystems and mercury emissions. | 26 | 27 | 28 |
29 / 05 / 2023We discussed water purification technologies, ditching of marshlands, processed waters and the fertilization of marshland forests. | 30 / 05 / 2023We went through the budget, talked about making a project reveal video and what was going on with human practises and collaborations. | 31 / 05 / 2023We discussed mercury in ecosystems, its impact, Minamata convention and eutrophication of bodies of water. We also got some very good advice concerning scientific research. |
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01 / 06 / 2023Joakim met with Linda Vuorijoki and they discussed about codons and how to harmonize them. Our team shirts arrived which were provided to us by Turku University of Applied Sciences. | 2 | 3 | 4 | |||
05 / 06 / 2023To celebrate World Environment Day, we published the collaboration video we organized. We asked various iGEM teams to tell us how they are saving this environment. Huge thank you to all the amazing teams that participated: iGEM Thrace, iGEM MIT-MAHE, iGEM Aix-Marseille, iGEM TU Braunschweig, iGEM UniCRETE and iGEM TU Dresden. We began our lab work! We started growing Escherichia coli (E. coli) culture to extract constructs. We prepared samples for P0, P1, P23S, P24S and P6. | 06 / 06 / 2023We prepared glycerol samples of P0, P1, P23S, P24S and P6. We also performed plasmid minipreps on the above-mentioned E. coli cultures. | 07 / 06 / 2023We prepared BG-11 medium for cyanobacteria. | 08 / 06 / 2023Discussed our ideas, the schedule and how are we preceding with the wiki building. | 09 / 06 / 2023We made our first liquid culture of cyanobacteria. | 10 | 11 |
12 / 06 / 2023We made Synechocystis sp. PCC6803 glycerol preps. We also grew more E. coli culture. | 13 / 06 / 2023Sara and Mariel double checked our implication grant application and then sent it. Talked about the upcoming Turku comic festival, organizing group funding with Kulttuurilahja and contacting press and other media. We filled out the village decision preference form. We made more E. coli minipreps. We also had a meeting with our primary assistant professor Pauli Kallio. | 14 / 06 / 2023We took group photos and individual photos with our new team shirts. We also filmed a part of the promo video. We posted our project reveal on our social media platforms: Instagram, Facebook, LinkedIn and TikTok. We had a research day when we planned our next experiments. | 15 / 06 / 2023Promo video team took a trip to a nearby marshland called Pomponrahka. We filmed parts of the promo video. We prepared competent E. coli cells for transformation. | 16 | 17 | 18 / 06 / 2023IT put up our project description on our wiki page. |
19 / 06 / 2023Our PI and Satu looked over our safety form and sent the preliminary version. We prepared antibiotic-free BG-11 growth plates with Synechocystis sp. PCC6803. We had our weekly lab meeting, too. | 20 / 06 / 2023We prepared our genes (P3 sYFP2 and P4 RBS S4) and then performed heat-shock transformation to competent E. coli cells. | 21 / 06 / 2023We made glycerol preps and minipreps of the transformed E. coli cultures (P3 sYFP2 and P4 RBS S4). | 22 / 06 / 2023We measured the DNA concentrations in our E. coli (P3 sYFP2 and P4 RBS S4) miniprep samples using NanoDrop. | 23 | 24 | 25 |
26 / 06 / 2023Weekly lab meeting. | 27 / 06 / 2023Talked about the upcoming Study in Turku event, Heureka collaboration and Aalto-Helsinki meet up. Lab team got everyone up to speed. We prepared LB and LBA media and grew E. coli cultures for P1, RBS S5 and EFE. Additionally, we had a meeting with our primary assistant professor Pauli Kallio. | 28 / 06 / 2023Discussed what to do, when and who is in charge. We also did team administration. We made glycerol preps and minipreps of EFE, sYFP2, RBS S4, RBS S5 and P1 E. coli cultures. | 29 / 06 / 2023We had a meeting with our primary assistant professor Pauli Kallio. | 30 |
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03 / 07 / 2023Weekly lab meeting. | 04 / 07 / 2023We started growing P0, P1 and RBS S4 E. coli cultures. | 05 / 07 / 2023We made glycerol preps and minipreps of P0, P1 and RBS S4 E. coli cultures and measured their DNA concentrations. We also made Synechocystis sp. PCC6803 preculture and E. coli cultures for P4 RBS S5, sYFP2, P2 RBS S3, P4 RBS S3 and P6 TT. | 06 / 07 / 2023We performed minipreps of P4 RBS S5, sYFP2, P2 RBS S3, P4 RBS S3 and P6 TT E. coli cultures. We also performed midipreps of promoter and backbone. | 07 / 07 / 2023We concentrated our DNA fragments by centrifugation. | 8 | 9 |
10 / 07 / 2023Discussed the trip to Paris and came up with team rules to keep up the work. Also some project administration. We performed an assembly reaction for sYFP2/EFE and merA/merB. | 11 / 07 / 2023Went through what is going on in every subteam and how to proceed. We also agreed on the team rules. Went through group funding status, talked about the upcoming HS Turku interview and how we would like to collaborate with the Edinburgh team. We performed a heat-shock transformation (sYFP2/EFE and merA/merB) to E. coli. | 12 / 07 / 2023We prepared an E. coli liquid culture and performed a colony PCR. | 13 / 07 / 2023Helsingin Sanomat Turku division came to our lab to interview us about our project and synthetic biology. We performed an assembly reaction for sYFP2/EFE and merA/merB. | 14 / 07 / 2023We performed a heat-shock transformation to E. coli and prepared agar plates. | 15 / 07 / 2023We made secondary E. coli cultures. | 16 |
17 / 07 / 2023We made new colonies and prepared colonies for a PCR reaction. | 18 / 07 / 2023Joakim made new gene order with added cutsites for cloning in pUC-19 We ran electrophoresis to analyze the results of PCR. | 19 / 07 / 2023We concentrated our DNA and ran a construct (P1, P2 RBS S3, P4 RBS S5 and P6 TT) assembly reaction. | 20 / 07 / 2023Discussed our implementation and who to contact to discuss our idea. We performed a RecBCD reaction and transformation to competent E. coli cells. | 21 / 07 / 2023We made secondary E. coli cultures for PCR. | 22 / 07 / 2023iGEM Calgary organized an event called JulyGEM in which we participated via recording. | 23 |
24 / 07 / 2023We invited Aalto-Helsinki team to Turku. We went sightseeing, gave them a tour of our lab and campus, ate in our student cafeteria and got to know each other. We had a conversation with a fisherman called Ari Savikko. He helped us understand the current situation of fish and our waters by telling us about artificial lakes Finland has and the way they have affected the environments. We performed a PCR reaction. | 25 / 07 / 2023We had a conversation with a fisherman called Jaakko Ikonen. We discussed the history of mercury, the current situation and how different things affect fish and fishers. Went through our current budget, upcoming events and wiki text status. We ran electrophoresis to analyze the results of PCR. | 26 / 07 / 2023Discussed about gene reorder in vectors and decided on the ordering of primers. Also talked about the next upcoming weeks of the lab. Discussed the Human Practice question upon the iGEM website and the vision about our work We had a lab meeting where we discussed gene reorder in vectors and ordering of primers. We also planned the next upcoming weeks in the lab. | 27 | 28 / 07 / 2023Mariel and Joakim represented the whole team at the Nordic iGEM Conference, NiC for short, and we won the conference! Judges were impressed by our presentation and we got rewarded with the golden pipette. The whole weekend was so much fun: getting to know different Nordic and Baltic teams and their project and learning a lot by listening to lectures and attending Hackathon. Thank you iGEM SDU for organizing this great event and thank you for all the teams that made this weekend so fun! | 29 / 07 / 2023Mariel and Joakim represented the whole team at the Nordic iGEM Conference, NiC for short, and we won the conference! Judges were impressed by our presentation and we got rewarded with the golden pipette. The whole weekend was so much fun: getting to know different Nordic and Baltic teams and their project and learning a lot by listening to lectures and attending Hackathon. Thank you iGEM SDU for organizing this great event and thank you for all the teams that made this weekend so fun! | 30 / 07 / 2023Mariel and Joakim represented the whole team at the Nordic iGEM Conference, NiC for short, and we won the conference! Judges were impressed by our presentation and we got rewarded with the golden pipette. The whole weekend was so much fun: getting to know different Nordic and Baltic teams and their project and learning a lot by listening to lectures and attending Hackathon. Thank you iGEM SDU for organizing this great event and thank you for all the teams that made this weekend so fun! |
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1 | 2 | 04 / 08 / 2023We discussed and went over feedback we had about team members. | 4 | 5 | 6 | |
07 / 08 / 2023We ran PCR to amplify our old gene fragments and our new merB gene (Pseudomonas aeruginosa). | 08 / 08 / 2023We talked about who is going to Paris and booked our tickets! Discussed the contribution, values and a possible field trip to a local lake and swamp area with an expert We made construct assemblies using new PCR amplified gene fragments: P0 pdF-C, P1 promoter, P2 RBS S3, P3 merA, P4 RBS S3, P5 merB, P6 terminator. | 09 / 08 / 2023We performed exonuclease reactions on the assemblies that we made the day before and transformed the assemblies into competent E. coli cells. | 10 | 11 / 08 / 2023We ran PCR with the construct assemblies that were made latest. | 12 / 08 / 2023We ran electrophoresis with the constructs that were amplified the day before. | 13 / 08 / 2023We made cultures of the E. coli cells that we transformed with the latest plasmid constructs. |
14 / 08 / 2023We performed colony PCR on transformed E. coli cells. We also made glycerol preps and midipreps of the cell cultures that were made the day before. Additionally, we started a Synechocystis sp. PCC6803 preculture. | 15 / 08 / 2023We planned a workshop and wiki home page Talked about what the presentation video is, the schedule and the division of the workload We run agarose gel electrophorensis with the PCR samples from the day before. We also started liquid cultures on transformed E. coli plate cultures. | 16 / 08 / 2023We had a conversation with Elisa Männistö about swamps, ecology and the role they have in our ecosystems. We gain a lot from the conversation. We run PCR on four different plasmid constructs. We also made minipreps and glycerol preps of merA and merB E. coli. Additionally, we had a lab meeting. | 17 / 08 / 2023We run electrophoresis with construct samples that were amplified with PCR. We also started the assembly of new constructs. | 18 / 08 / 2023We met with Laura to discuss the protection of the Baltic Sea, what things to consider in these types of projects and how our project can have a continuous impact on our waters. We received a lot of great points and questions we need to find answers to. We performed a RecBCD reaction to the new constructs that were started the day before. Then we transformed competent E. coli cells with the constructs. | 19 | 20 |
21 / 08 / 2023We started a new Synechocystis main culture. | 22 / 08 / 2023We planned the Heureka workshops and Science Basement talk we are doing as a collaboration with Aalto-Helsinki. Unfortunately Aalto-Helsinki could not make it to the meeting but we got a lot of work done and sent our ideas to them. Went through our project budget and collaboration plans. We performed colony PCR on transformed E. coli cells. Then we run electrophoresis on the PCR samples. | 23 / 08 / 2023We measured concentrations of our gene fragments and prepared LBA for our cell cultures. | 24 | 25 / 08 / 2023We started the assembly of new constructs by preparing a fragment mastermix. | 26 | 27 |
28 / 08 / 2023The lab team researched, planned and contacted experts about mercury and how to handle it responsibly. We performed a RecBCD reaction to the new constructs that were started the day before. Then we transformed competent E. coli cells with the constructs. We also made cultures for P2 RBS S4 and P2 RBS S3. | 29 / 08 / 2023We visited a local underground wastewater treatment plant. We took a tour of their facilities, learned about their work and had a meeting with an expert to discuss biological purification and what to consider in wastewater treatment. Talked about future events and went through every subteams status. We prepared new LBA and started cultures for P2 RBS S4 and P2 RBS S3 again. | 30 / 08 / 2023We had a stand about our team, project and synthetic biology at the fair for new students of Turku. We met numerous new students, guided them with pipetting and got to answer and ask interesting questions. We made transformation with our constructs to competent E. coli cells. | 31 / 08 / 2023We had a Zoom meeting about our collaboration and decided to do a report about cyanobacteria and biosafety. Also we discussed our projects and shared feelings about the slow growth rate of Synechocystis. We plated E. coli colonies to LBA plates with spectinomycin to choose cells with transformed plasmids. |
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01 / 09 / 2023We performed colony PCR on transformed E. coli cells. Then we run electrophoresis on the PCR samples. | 02 / 09 / 2023We organized a DNA, RNA & Protein workshop in Heureka, a science center in Vantaa, with the Aalto-Helsinki team. In the workshop the visitors learned about the power trio of life and got to build DNA & RNA themselves. | 3 | ||||
04 / 09 / 2023We made plasmid preparations of P2 RBS S3 and PDF-C backbone, and measured the concentrations. | 05 / 09 / 2023We talked about what Katja and the Turun pursiseura does, the insights Turun pursiseura has regarding sailing and the Baltic Sea and then how we should consider them in our project. Went through collaborations and lab stuff. We started the assembly of six new constructs. | 06 / 09 / 2023We met our PI Pauli Kallio and discussed our implementation text and how to present our ideas the best way with visual aids. We performed a RecBCD reaction to the new constructs that were started the day before. | 07 / 09 / 2023The Team Leaders discussed what has to be done and with what urgency. We did a delegation and planned the next steps of our project. We transformed our latest assemblies into competent E. coli cells. | 08 / 09 / 2023We talked about the restoration of peatlands, what Hiilipörssi does and what things we should take into consideration regarding restoration in our project. We plated colonies that were transformed with our latest plasmid constructs the day before. | 9 | 10 |
11 / 09 / 2023We went over the first versions of our lab wiki texts with our advisors. They gave us really good comments and insights. We will take these points under consideration. We went over the first versions of our Human Practice and other wiki texts with our advisors. They gave us really good comments and insights. We will take these points under consideration. We performed colony PCR reaction with cells that were transformed with our latest plasmid constructs. We also started a new Synechocystis sp. PCC803 main culture. | 12 / 09 / 2023We went through our current budget and talked about the lab team's future plans. | 13 / 09 / 2023We discussed the overall vision for the presentation video and after informed the rest of the presentation video group about it. | 14 | 15 | 16 / 09 / 2023We organized a DNA, RNA & Protein workshop in Heureka, a science center in Vantaa, with the Aalto-Helsinki team. In the workshop the visitors learned about the power trio of life and got to build DNA & RNA themselves. The second time we added pipetting to the workshop in order to be more interactive and exciting. | 17 |
18 | 19 / 09 / 2023We talked about our achievements, how they should be reported and how we should continue in the lab. In addition we ate apple pie that our team leader Sara had made and talked about this journey we have faced. Short meeting, went through our plans regarding upcoming Science Basement talk | 20 / 09 / 2023We filmed the Design, Education, Ending and Introduction scenes. | 21 / 09 / 2023We filmed the Human Practice, Methodology, Safety and Next steps scenes. Went over the Human Practice and other wiki texts. Discussed animal rights and our project goals We made another colony PCR with cells that were transformed with our latest plasmid constructs. | 22 / 09 / 2023We made elektrophoresis analysis on the PCR samples from the day before to see if the merA-merB plasmid assembly worked. | 23 | 24 |
25 / 09 / 2023We visited a local wellness center to teach their customers about the basics of synthetic biology. We held a presentation with interactive discussion sessions and at the end showed our everyday laboratory equipment. | 26 / 09 / 2023Went through wiki and each pages status. | 27 / 09 / 2023We met with the GU-Frankfurt team via Zoom. We discussed our projects and wastewater treatment in our countries. It was wonderful to share our thoughts and hear how other iGEM projects are going. | 28 | 29 | 30 / 09 / 2023We gave an afternoon talk about bioremediation with our collaborating team at Helsinki main library Oodi. The talk can be found here |
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2 | 03 / 10 / 2023Went through wiki status and budget. Talked about our plans for Paris. | 4 | 05 / 10 / 2023We planned to film our Results scenes. We performed colony PCR using three colonies from a plate containing E. coli cells with merA/merB constructs. After running the PCR, we made gel electrophoresis with the samples to analyze their content. | 06 / 10 / 2023We made minipreps from the three merA/merB colonies from the day before. | 7 | 8 |
9 | 10 / 10 / 2023Went through wiki and talked about our plans for the Jamboree. | 11 | 12 | 13 | 14 / 10 / 2023We will attend Turku’s Comic Festival and the idea is to have an interactive workshop that mixes science and art. | 15 |
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23 | 24 / 10 / 2023Planned team meeting | 25 | 26 | 27 | 28 | 29 |
30 | 31 / 10 / 2023Planned team meeting |
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1 | 02 / 11 / 2023First day of the Jamboree | 03 / 11 / 2023Jambree day 2 | 04 / 11 / 2023Jamboree day 3 | 05 / 11 / 2023Final day of the Jamboree | ||
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