Parts
Basic Parts
| name | Description |
|---|---|
| BBa_K4803000 | gp130(IL6Rβ) extracellular domain |
| BBa_K4803001 | gp80(IL6Rα) extracellular domain |
| BBa_K4803002 | IFNGR extracellular domain |
| BBa_K4803003 | FRB |
| BBa_K4803004 | FKBP |
| BBa_K4803005 | CD4 signal |
| BBa_K4803006 | CD28 transmembrane domain |
| BBa_K4803007 | STAT3 responsive element |
| BBa_K4803008 | AP-1 responsive element |
| BBa_K4803010 | mini promoter |
| BBa_K4803011 | TRE 3G promoter |
| BBa_K4803012 | 3xFCS |
| BBa_K4803013 | 3xPPVCS |
| BBa_K4803014 | KKYL |
| BBa_K4803015 | TVMVThr-AI_Stable |
| BBa_K4803016 | TVMVThr-AI_Disorder |
Composite Parts
| name | Description |
|---|---|
| BBa_K4803100 | TRE3G-ZsGrren |
| BBa_K4803101 | CD4signal-gp130ex-CD28TMD-TEVp |
| BBa_K4803102 | CD4signal-gp80ex-CD28TMD-TEV CS-tTA |
| BBa_K4803103 | CD4signal-gp80ex-CD28TMD-TEVp |
| BBa_K4803104 | CD4signal-gp130ex-CD28TMD-TEV CS-tTA |
| BBa_K4803105 | CD4signal-IFNGRex-CD28TMD-TEVp |
| BBa_K4803106 | CD4signal-IFNGRex-CD28TMD-TEV CS-tTA |
| BBa_K4803107 | CD4signal-IFNGRex-CD28TMD-TEV CS-PPVp |
| BBa_K4803108 | CD4signal-FKBP-CD28TMD-TEVp |
| BBa_K4803109 | CD4signal-FRB-CD28TMD-TEV CS-tTA |
| BBa_K4803111 | CD4signal-eGFP-FCS-CD28TMD-PPVcs-KKYL |
| BBa_K4803113 | FCS-CD28TMD-PPVcs-KKYL |
| BBa_K4803117 | AP-1 responsive element-mini promoter-ZsGreen |
| BBa_K4803118 | STAT3 responsive element-mini promoter-ZsGreen |
Part Collection
Our Parts Collection consists of a total of nine parts, which are divided into three units: Detection Unit, Amplification Unit, and Secretion Unit.
Detection Unit, Amplification Unit, and Secretion Unit are the key components of SWIFT.
Beyond our project, these parts will be of reference and help to many iGEM teams who want to use SWIFT in situations that require rapid protein secretion after ligand detection.
Detection Unit
In the Detection Unit, we proved that MESA works even if the extracellular domain of MESA is changed, and showed the connection to the Amplification Unit and the Secretion Unit by using protease as the POI to be released from MESA.
BBa_K4803105
CD4signal-IFN-γRex-CD28TMD-TEVp
composite
This is a protease chain of MESA and detects IFN-γ along with BBa_K4803106 or BBa_K4803107.
BBa_K4803106
CD4signal-IFNGRex-CD28TMD-TEV CS-tTA
composite
This is a target chain of MESAt and when transfected into cells together with BBa_K4803105, tTA is released when IFN-γ is detected.
BBa_K4803107
CD4signal-IFNGRex-CD28TMD-TEV CS-PPVp
composite
This is a target chain of MESA and when transfected into cells together with BBa_K4803105, PPV protease is released when IFN-γ is detected.
PPV protease can be substituted with other protease.
BBa_K4803108
CD4signal-FKBP-CD28TMD-TEVp
composite
This is a protease chain of MESA and detects rapamycin along with BBa_K4803109.
BBa_K4803109
CD4signal-FRB-CD28TMD-TEV CS-tTA
composite
This is a target chain of MESA and when transfected into cells together with BBa_K4803108, tTA is released when rapamycin is detected.
Amplification Unit
In the Amplification Unit, an example of an Amplification Part was shown using TVMV protease and a linker part with a Threonine protease cleavage site.
In this Unit, two parts are registered according to the stability by 3D structure simulation, and it is shown that an Amplification System with various proteases can be made based on this Part.
BBa_K4803015
TVMVThr-AI_Stable basic
This Part consists of TVMV protease, Thr-cleavage site, and Inhibitor of TVMV protease linked by a linker.
This is the sequence with the highest cleavage site stability among those whose stability was evaluated by simulation.
BBa_K4803016
TVMVThr-AI_Disorder
basic
This Part consists of TVMV protease, Thr-cleavage site, and Inhibitor of TVMV protease linked by a linker.
This is the sequence with the lowest cleavage site stability among those whose stability was evaluated by simulation.
Secretion Unit
In the Secretion Unit, parts to build a system for secreting POI by the protease of Amplification Unit are described. By designing the protein of interest and the protease cleavage site, you are free to use SWIFT as you wish!
BBa_K4803111
CD4signal-eGFP-FCS-CD28TMD-PPVcs-KKYL
composite
This part secretes eGFP when the ER retention signal, KKYL, is removed by PPV protease.
BBa_K4803113
FCS-CD28TMD-PPVcs-KKYL
composite
By adding the protein of interest upstream of FCS, it is possible to secrete the protein of interest in the presence of PPV protease.