Parts Library
| Part Name | Registry ID | Part Type | Status | Length (bp) | Description |
|---|---|---|---|---|---|
| Lumbrokinase (NCBI AY187629) | BBa_K4673017 | Basic, DNA | New, Utilized | 741 | The gene that produce Lumbrokinase |
| T7 terminator | BBa_B0012 | Basic, RNA | Existing | 41 | Terminates RNA transcription |
| 6x his-tag | BBa_K4673028 | Basic, tag | New, Utilized | 18 | The presence of 6X his-tag can be detected by antibodies |
| pelB signal sequence | BBa_J32006:Design | Basic, DNA | Existing | 1774 | The signal peptide, promoting protein secretion |
| RBS | BBa_J100320:Design | Basic | Existing | 735 | Ribosomal binding site, providing location where ribosomes bind |
| T7 promoter | BBa_I72074 | Basic, promoter | Existing | 46 | Binding site for RNA polymerase |
| lacI promoter | BBa_R0010 | Basic, promoter | Existing | 200 | Promoter region for LacI gene |
| lacI | BBa_K4673026 | Basic, repressor | New, Utilized | 1083 | LacI is a regulated repressor protein |
| ROP | BBa_K2259010 | Basic, protein | Existing | 189 | Regulate the copy number of plasmids in E. coli |
| ori | BBa_J01002 | Basic, conjunction | Existing | 365 | Replication origin for plasmids |
| Ampicillin resistance | BBa_P1002 | Basic, DNA | Existing | 943 | Allowing colonies to survive under an ampicillin-containing environment and helping us to identify the results in blue-white screening. |
| FadR regulator | BBa_K4673025 | Basic, regulatory | New, Utilized | 32 | The FadR regulator controls the fad gene |
| FadH operator | BBa_K4673021 | Basic, operator | New | 32 | FadH operator is the binding site for FadR |
| LacZα | BBa_K4673027 | Basic, reporter | New, Utilized | 149 | LacZα is a common reporter gene within E. coli |
| β-lactamase coding region | BBa_K4673031 | Basic, DNA | New, Utilized | 861 | β-lactamase coding region is an ampicillin resistance gene |
| AmpR promoter | BBa_K4673029 | Basic, regulatory | New, Utilized | 105 | AmpR promoter is a promoter for ampicillin resistance |
| lac promoter | BBa_K4673022 | Basic, promoter | New, Utilized | 30 | Lac promoter binds RNA polymerase for transcription |
| lac operon | BBa_K4673018 | Basic, regulatory | New, Utilized | 230 | Lac operon involved in lactose metabolism |
| lac operator | BBa_K4673024 | Basic, operator | New, Utilized | 25 | Operator is where the repressor binds to the operon |