IPTG Induction

“Expression, purification, and characterization of recombinant lumbrokinase PI239 in Escherichia coli” is a study done by Zhe-rong Xu, Yun-mei Yan, Qi-feng Gui, Li-na Zhang, and Lin Hu, who were undergraduates in Zhejiang University in 2009. This study focused on transferring lumbrokinase producing DNA from earth worms and insert this DNA into plasmids of E.coli BL21 to achieve production of lumbrokinase when induced by Isopropyl ß-D-1-thiogalactopyranoside (IPTG). This study succeeded in inserting the lumbrokinase DNA into plasmid pET-22b-rLK, which is then transformed into E.coli BL21 so that the modified E.coli BL21 produces lumbrokinase when induced by IPTG. This journal article is important to our project because it provided us the basics of our project, which is expression of lumbrokinase induced by IPTG, so that our team can further modify the induction mechanism given that IPTG induction is confirmed working.