A 7-methylxanthine compound that has important applications in the treatment of myopia.
7-Methylxanthine
a gene that exists in E.Coli that is important for biosynthesis of amino acids and bacterial cell wall formation. We knock out the gene and reintroduce it to the bacterial chromosome through plasmid to ensure that only cells that contain the plasmid will survive.
Asd Gene Plasmid
An antibiotic often produced by bacteria to target competing bacteria strain. In this project, Colicin M was the main bacteriocin involved in treating Bovine Mastitis.
Bacteriocin
A biobrick that is the main precursor to the genes used to synthesize golden rice. It aims to produce eventually Colicin M, which is a type of Bacteriocin.
BBa_K1931009
A biobrick that is the main precursor to the genes used to synthesize golden rice. It aims to produce eventually Beta-Carotene, the precursor to Vitamin A.
BBa_K2151200
Biobrick containing the gene necessary for the production of the enzyme NdmA.
BBa_K734000 (NdmA)
Biobrick containing the gene necessary for the production of the enzyme NdmB
BBa_K734000 (NdmB)
A carotenoid used to synthesize Vitamin A in the body.
Beta Carotene
Compound of the methylxanthine class that can be used as a starting point for the synthesis of 7-methylxanthines.
Caffeine
a biobrick that is a precursor/gene for the product Lead it Go which allows for selective lead uptake.
CBP4
A bacteria with an Lldr Regulatory protein that when bound to lactate, Lldr allows RNA Polymerase to bind and transcribe the downstream gene. When not bound to lactate, LldR represses transcription.
Cell that can Transcribe Downstream Genes
a biobrick that is a precursor/gene for the product Lead it Go which allows for metal binding
crs5
a bacterial cell that expresses the Asd gene which can form amino acids and the bacterial cell wall important for survival of bacteria
E. coli Cells with Plasmid
This is a genetically modified E.Coli bacteria that can uptake mercury. It is produced by combining the MB E.Coli and Mercury Uptake plasmid.
E. coli Uptakes Mercury
This is a genetically modified E.Coli bacteria that can uptake nickel and lead. It is produced by combining the MB E.Coli and the Nickel and Lead Uptake plasmid
E. coli Uptakes Nickel and Lead
a model cancer bacteriotherapy that is used to detect, monitor, and mitigate breast cancer. This bacteria will be injected into the site of the tumor and grow in cancer cells, weakening and destroying cancer cells. This bacteria also allows the patient to monitor the primary tumor via a fluorescent imaging device
E. coli with Kill Switch
Bacteria; often used as a model organism when conducting synthetic biology experiments/projects.
E. Coli
A metabolic intermediate in Beta-Carotene synthesis.
Farnesyl Pyrophosphate
.Another intermediate precursor in biosynthesis to existing carotenoids.
Geranylgeranyl Pyrophosphate
Genetically engineered rice designed to be high in Vitamin A. It was supposed to be used to address Vitamin A deficiencies in many places of the world, including Africa and South Asia.
Golden Rice
creates holes in the bacterial cell membrane and cuases lysis. To prevent unnecessary lysis, antiholin (a protein) binds to holin to inactivate it.
Holin Kill Switch
An intermediate precursor to many existing carotenoids, including Beta-Carotene.
Isopentanyl Diphosphate
This is a bacterial strain that will simultaneously express heavy metal transport proteins and metallotheioneins to combat heavy metal pollution problems (especially in water)
Lead it Go
a regulatory protein used to control expression of downstream genes.
LldR Protein with Operon
a model cancer bacteriotherapy that is used to detect, monitor, and mitigate breast cancer. This bacteria will be injected into the site of the tumor and grow in cancer cells, weakening and destroying cancer cells. This bacteria also allows the patient to monitor the primary tumor via a fluorescent imaging device
Lumiderm
An enzyme involved in converting lycopene to Beta-Carotene.
Lycopene Betacyclase
A carotenoid involved in Beta-Carotene synthesis. It is an antioxidant and can be found in tomatoes and watermelon.
Lycopene
red fluorescent protein that bacterias can express in order to track them.
Mcardinal
a plasmid created by fusing together the merT and merP biobricks. Its used to uptake mercury
Mercury Uptake
a biobrick that is a precursor/gene for the product Lead it Go that transcribes for a transporter that brings mercury into the cytoplasm of the cell.
Merp
a biobrick that is a precursor/gene for the product Lead it Go. It encodes for a transmembrane protein that assists in transporting Hg(II) into the cytoplasm of the bacterial cell
Mert
a genetically modified bacterium which binds metals. Without this precursor, the bacteria will not be able to uptake metals. It is produced by inserting the crs5 gene into the E.Coli’s genetic information.
Metal Binding E. coli
Key enzyme in the production pathway of 7-methylxanthines, particularly in the production of 7-methylxanthine.
NdmA
Key enzyme in the production pathway of 7-methylxanthines, particularly in the production of paraxanthine.
NdmB
a plasmid created by fusing together nixA and cbp4 biobricks. It is ues for nickel and lead uptake
Nickel and Lead
a bio brick that is a precursor/gene for the product Lead it Go which allows the bacterial cell to absorb nickel ions
Nixa
A 7-methylxanthine compound that has applications as dietary supplements to increase muscle mass and wakefulness.
Paraxanthine
An enzyme specific to phytoene involved in converting phytoene to lycopene.
Phytoene Desaturase
The true first step in carotenoid synthesis in plants.
Phytoene
a bacterial cell that will express a red fluorescence that can then be used to track the bacterium throughout the body
Red Fluorescent Cell
a protein that is used to cleave DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end
Restriction Enzyme
This is a bacterial strain that will simultaneously express heavy metal transport proteins and metallotheioneins to combat heavy metal pollution problems (especially in water).
Super E. coli
A 7-methylxanthine that is an important intermediate in the synthesis of 7-methylxanthine.
Theobromine