Biology Protocols

In this section, you will find all the protocols that we used during our time in the labs.
Please click through the tabs below to look at each of our protocols:
  • Stock solutions for creating competent bacteria
  • Antibiotic Stock Solutions
  • Pouring agar Plates
  • Preparing Cells
  • Making Competent Cells
  • E. coli Transformation
  • Determination of bacterial optical density (OD) using the spectrophotometer
  • Conducting Gel Electrophoresis
  • PCR Preparations

  1. LB medium 200 ml in 1L flask (2g Tryptone, 1g Yeast extract, 1g NaCl and H₂O to 200ml). Close with sponge and foil. Label, autoclave at 125°C for 15 minutes and store at room temperature.
  2. LB agar 2 x 50ml in 125ml flask: (500mg Tryptone, 250mg Yeast extract, 250mg NaCl, 750mg Agar and H₂O to 50ml). Close with sponge and foil. Label, autoclave at 125°C for 15 minutes and store at room temperature.
  3. 0.1 M MgCl₂ solution (2.033 g of MgCl₂·6H₂O in 100ml of H₂O) in 100ml Pyrex bottle. Autoclave and store at 4°C.
  4. 0.1 M CaCl₂ solution (11 g of CaCl₂·6H₂O in 500 ml of H₂O) in 500ml Pyrex bottle. Autoclave and store at 4°C.
  5. 50% glycerol solution (5ml of glycerol and 5ml H₂O in 20ml) culture tube with screw cup. Autoclave and store at 4°C.